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minimum essential medium non-essential amino acids (mem neaa)  (Thermo Fisher)


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    Thermo Fisher minimum essential medium non-essential amino acids (mem neaa)
    Minimum Essential Medium Non Essential Amino Acids (Mem Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimum+essential+medium+non-essential+amino+acids+(mem+neaa)/minimum+essential+medium+non+essential+amino+acids/pmc12152109-74-1-9
    Average 90 stars, based on 1 article reviews
    minimum essential medium non-essential amino acids (mem neaa) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Formulation:

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury–like inflammation
    Article Snippet: For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAX TM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml Sigma-Aldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury-like inflammation.
    Article Snippet: Co‐cultures and monocultures For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAXTM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml SigmaAldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: Inhibition of CD226 co-stimulation suppresses diabetes development in the NOD mouse by augmenting regulatory T cells and diminishing effector T cell function.
    Article Snippet: Cell suspensions were washed with complete RPMI (cRPMI; RPMI 1640 media Phenol Red with l-Glutamine 139 [Lonza, Basel, CH-BS, Switzerland], 5 mmol/l HEPES [Gibco, Waltham, MA, USA], 5 mmol/l Minimum essential medium 140 Non-Essential Amino Acids [MEM NEAA; Gibco], 2 mmol/l Glutamax [Gibco], 50 μg/ml penicillin 141 [Gibco], 50 μg/ml streptomycin [Gibco], 20 mmol/l sodium pyruvate [Gibco], 50 mmol/l 2-mercaptoethanol [Sigma-Aldrich, St Louis, MO, USA], 20 mmol/l sodium hydroxide [Sigma-Aldrich] and 10% FBS [Genesee Scientific, El Cajon, CA, USA]) and pelleted by centrifugation (350 × g for 7 min).

    Article Title: Proliferation Assay Using Cryopreserved Porcine Peripheral Mononuclear Cells Stimulated With Concanavalin A and Analyzed With FCS Express TM 7.18 Software
    Article Snippet: 100× minimum essential medium non-essential amino acids (MEM NEAA) (Thermo Fisher Scientific, Gibco ® , catalog number: 11140050); stored at 4 °C 11.

    Article Title: Bone sialoprotein stimulates cancer cell adhesion through the RGD motif and the αvβ3 and αvβ5 integrin receptors
    Article Snippet: MDA-MB-231 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies, Ltd.), supplemented with 10% heat-inactivated fetal calf serum (FCS; Biochrom GmbH), 1% penicillin-streptomycin (P/S; Sigma-Aldrich; Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Sigma-Aldrich; Merck KGaA), 5 ml Minimum Essential Medium non-essential amino acids (MEM NEAA), 100X (Gibco; Thermo Fisher Scientific, Inc.) and 1 mM sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.).

    Article Title: UFMylation promotes orthoflavivirus infectious particle production
    Article Snippet: Huh7 cells, Huh7.5 cells, A549 cells, Vero cells, and 293T cells were grown in Dulbecco’s modification of Eagle’s medium (DMEM; Mediatech) supplemented with 10% fetal bovine serum (HyClone), 1X minimum essential medium non-essential amino acids (Thermo Fisher), and 25 mM HEPES (Thermo Fisher), referred to as complete DMEM (cDMEM).

    Article Title: Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases
    Article Snippet: Minimum Essential Medium (MEM) Non-essential Amino Acids (NEAA) (1×) 2.5 mL (Life Technologies Corporation; Grand Island, NY, USA) , MEM NEAA (1X) 2.5 mL.

    Knock-Out:

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury–like inflammation
    Article Snippet: For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAX TM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml Sigma-Aldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury-like inflammation.
    Article Snippet: Co‐cultures and monocultures For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAXTM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml SigmaAldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: Inhibition of CD226 co-stimulation suppresses diabetes development in the NOD mouse by augmenting regulatory T cells and diminishing effector T cell function.
    Article Snippet: Cell suspensions were washed with complete RPMI (cRPMI; RPMI 1640 media Phenol Red with l-Glutamine 139 [Lonza, Basel, CH-BS, Switzerland], 5 mmol/l HEPES [Gibco, Waltham, MA, USA], 5 mmol/l Minimum essential medium 140 Non-Essential Amino Acids [MEM NEAA; Gibco], 2 mmol/l Glutamax [Gibco], 50 μg/ml penicillin 141 [Gibco], 50 μg/ml streptomycin [Gibco], 20 mmol/l sodium pyruvate [Gibco], 50 mmol/l 2-mercaptoethanol [Sigma-Aldrich, St Louis, MO, USA], 20 mmol/l sodium hydroxide [Sigma-Aldrich] and 10% FBS [Genesee Scientific, El Cajon, CA, USA]) and pelleted by centrifugation (350 × g for 7 min).

    Article Title: Proliferation Assay Using Cryopreserved Porcine Peripheral Mononuclear Cells Stimulated With Concanavalin A and Analyzed With FCS Express TM 7.18 Software
    Article Snippet: 100× minimum essential medium non-essential amino acids (MEM NEAA) (Thermo Fisher Scientific, Gibco ® , catalog number: 11140050); stored at 4 °C 11.

    Article Title: Bone sialoprotein stimulates cancer cell adhesion through the RGD motif and the αvβ3 and αvβ5 integrin receptors
    Article Snippet: MDA-MB-231 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies, Ltd.), supplemented with 10% heat-inactivated fetal calf serum (FCS; Biochrom GmbH), 1% penicillin-streptomycin (P/S; Sigma-Aldrich; Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Sigma-Aldrich; Merck KGaA), 5 ml Minimum Essential Medium non-essential amino acids (MEM NEAA), 100X (Gibco; Thermo Fisher Scientific, Inc.) and 1 mM sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.).

    Article Title: UFMylation promotes orthoflavivirus infectious particle production
    Article Snippet: Huh7 cells, Huh7.5 cells, A549 cells, Vero cells, and 293T cells were grown in Dulbecco’s modification of Eagle’s medium (DMEM; Mediatech) supplemented with 10% fetal bovine serum (HyClone), 1X minimum essential medium non-essential amino acids (Thermo Fisher), and 25 mM HEPES (Thermo Fisher), referred to as complete DMEM (cDMEM).

    Article Title: Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases
    Article Snippet: Minimum Essential Medium (MEM) Non-essential Amino Acids (NEAA) (1×) 2.5 mL (Life Technologies Corporation; Grand Island, NY, USA) , MEM NEAA (1X) 2.5 mL.

    Multiple Displacement Amplification:

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury–like inflammation
    Article Snippet: For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAX TM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml Sigma-Aldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury-like inflammation.
    Article Snippet: Co‐cultures and monocultures For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAXTM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml SigmaAldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: Inhibition of CD226 co-stimulation suppresses diabetes development in the NOD mouse by augmenting regulatory T cells and diminishing effector T cell function.
    Article Snippet: Cell suspensions were washed with complete RPMI (cRPMI; RPMI 1640 media Phenol Red with l-Glutamine 139 [Lonza, Basel, CH-BS, Switzerland], 5 mmol/l HEPES [Gibco, Waltham, MA, USA], 5 mmol/l Minimum essential medium 140 Non-Essential Amino Acids [MEM NEAA; Gibco], 2 mmol/l Glutamax [Gibco], 50 μg/ml penicillin 141 [Gibco], 50 μg/ml streptomycin [Gibco], 20 mmol/l sodium pyruvate [Gibco], 50 mmol/l 2-mercaptoethanol [Sigma-Aldrich, St Louis, MO, USA], 20 mmol/l sodium hydroxide [Sigma-Aldrich] and 10% FBS [Genesee Scientific, El Cajon, CA, USA]) and pelleted by centrifugation (350 × g for 7 min).

    Article Title: Proliferation Assay Using Cryopreserved Porcine Peripheral Mononuclear Cells Stimulated With Concanavalin A and Analyzed With FCS Express TM 7.18 Software
    Article Snippet: 100× minimum essential medium non-essential amino acids (MEM NEAA) (Thermo Fisher Scientific, Gibco ® , catalog number: 11140050); stored at 4 °C 11.

    Article Title: Bone sialoprotein stimulates cancer cell adhesion through the RGD motif and the αvβ3 and αvβ5 integrin receptors
    Article Snippet: MDA-MB-231 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies, Ltd.), supplemented with 10% heat-inactivated fetal calf serum (FCS; Biochrom GmbH), 1% penicillin-streptomycin (P/S; Sigma-Aldrich; Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Sigma-Aldrich; Merck KGaA), 5 ml Minimum Essential Medium non-essential amino acids (MEM NEAA), 100X (Gibco; Thermo Fisher Scientific, Inc.) and 1 mM sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.).

    Article Title: UFMylation promotes orthoflavivirus infectious particle production
    Article Snippet: Huh7 cells, Huh7.5 cells, A549 cells, Vero cells, and 293T cells were grown in Dulbecco’s modification of Eagle’s medium (DMEM; Mediatech) supplemented with 10% fetal bovine serum (HyClone), 1X minimum essential medium non-essential amino acids (Thermo Fisher), and 25 mM HEPES (Thermo Fisher), referred to as complete DMEM (cDMEM).

    Article Title: Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases
    Article Snippet: Minimum Essential Medium (MEM) Non-essential Amino Acids (NEAA) (1×) 2.5 mL (Life Technologies Corporation; Grand Island, NY, USA) , MEM NEAA (1X) 2.5 mL.

    Cell Culture:

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury–like inflammation
    Article Snippet: For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAX TM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml Sigma-Aldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: A new human autologous hepatocyte/macrophage co-culture system that mimics drug-induced liver injury-like inflammation.
    Article Snippet: Co‐cultures and monocultures For co-cultures PHH were seeded in a ratio of 4:1 on the respective macrophage monoculture and for the PHH monoculture the respective share of PHH were seeded in Williams E medium with GlutaMAXTM (cat. no. 32551087, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) with fetal calf serum (FCS; cat. no. S0615-500 ml SigmaAldrich, St. Louis, USA), insulin (cat. no. PZN: 02526396, Lilly, Germany), dexamethasone (cat. no. PZN: 08704404, Jenapharm/MIBE GmbH, Germany), HEPES (cat. no. 15630–056, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), sodium pyruvate (cat. no. 11360–039, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), minimum essential medium non-essential amino acids (MEM-NEAA; cat. no. 11140–035, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and penicillin/streptomycin (cat. no. 15140122, Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA).

    Article Title: Inhibition of CD226 co-stimulation suppresses diabetes development in the NOD mouse by augmenting regulatory T cells and diminishing effector T cell function.
    Article Snippet: Cell suspensions were washed with complete RPMI (cRPMI; RPMI 1640 media Phenol Red with l-Glutamine 139 [Lonza, Basel, CH-BS, Switzerland], 5 mmol/l HEPES [Gibco, Waltham, MA, USA], 5 mmol/l Minimum essential medium 140 Non-Essential Amino Acids [MEM NEAA; Gibco], 2 mmol/l Glutamax [Gibco], 50 μg/ml penicillin 141 [Gibco], 50 μg/ml streptomycin [Gibco], 20 mmol/l sodium pyruvate [Gibco], 50 mmol/l 2-mercaptoethanol [Sigma-Aldrich, St Louis, MO, USA], 20 mmol/l sodium hydroxide [Sigma-Aldrich] and 10% FBS [Genesee Scientific, El Cajon, CA, USA]) and pelleted by centrifugation (350 × g for 7 min).

    Article Title: Proliferation Assay Using Cryopreserved Porcine Peripheral Mononuclear Cells Stimulated With Concanavalin A and Analyzed With FCS Express TM 7.18 Software
    Article Snippet: 100× minimum essential medium non-essential amino acids (MEM NEAA) (Thermo Fisher Scientific, Gibco ® , catalog number: 11140050); stored at 4 °C 11.

    Article Title: Bone sialoprotein stimulates cancer cell adhesion through the RGD motif and the αvβ3 and αvβ5 integrin receptors
    Article Snippet: MDA-MB-231 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Life Technologies, Ltd.), supplemented with 10% heat-inactivated fetal calf serum (FCS; Biochrom GmbH), 1% penicillin-streptomycin (P/S; Sigma-Aldrich; Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Sigma-Aldrich; Merck KGaA), 5 ml Minimum Essential Medium non-essential amino acids (MEM NEAA), 100X (Gibco; Thermo Fisher Scientific, Inc.) and 1 mM sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.).

    Article Title: UFMylation promotes orthoflavivirus infectious particle production
    Article Snippet: Huh7 cells, Huh7.5 cells, A549 cells, Vero cells, and 293T cells were grown in Dulbecco’s modification of Eagle’s medium (DMEM; Mediatech) supplemented with 10% fetal bovine serum (HyClone), 1X minimum essential medium non-essential amino acids (Thermo Fisher), and 25 mM HEPES (Thermo Fisher), referred to as complete DMEM (cDMEM).

    Article Title: Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases
    Article Snippet: Minimum Essential Medium (MEM) Non-essential Amino Acids (NEAA) (1×) 2.5 mL (Life Technologies Corporation; Grand Island, NY, USA) , MEM NEAA (1X) 2.5 mL.



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    Image Search Results


    EB media formulation for hiPSCs.

    Journal: International Journal of Molecular Sciences

    Article Title: Optimized Prime Editing of Human Induced Pluripotent Stem Cells to Efficiently Generate Isogenic Models of Mendelian Diseases

    doi: 10.3390/ijms26010114

    Figure Lengend Snippet: EB media formulation for hiPSCs.

    Article Snippet: Minimum Essential Medium (MEM) Non-essential Amino Acids (NEAA) (1×) 2.5 mL (Life Technologies Corporation; Grand Island, NY, USA) , MEM NEAA (1X) 2.5 mL.

    Techniques: Formulation, Knock-Out